SOLUTION
AND
PRODUCTION INSTRUCTIONS
FOR
MICROSCOPIC DYES
1) Alum carmine, Grenacher
5 g alum carmine (1A-112) are dissolved in 100 cc of distilled water by boiling. After cooling, filter and add a few drops of carbolic acid for preservation.
2) Alum carmine, conc. Grenacher
12 g alum carmine (1A-112) are dissolved in 100 cc of distilled water by boiling for a long time. After cooling, top up to the original amount of liquid and filter.
3) Aldehyde fuchsin, Gomori administration
A. Stock solution
0.750 g of aldehyde fuchsin (1A-360) are dissolved in 100 cc of 70% ethyl alcohol by shaking and filtered (shelf life at least 1 year).
B. Working solution
Mix 25 cc of stock solution with 75 cc of 70% ethyl alcohol and 1 cc of 98% acetic acid (shelf life several months).
4) Alizarinviridin-chrome alum
5 g of alizarinviridin-chrome alum (1A-382) are dissolved by boiling in 100 cc of distilled water and the cooled solution is filtered.
5) Ammonia carmine, Hoyer
To prepare a concentrated solution, boil 3 g of ammonia carmine (1A-456) in 100 cc of distilled water and filter after cooling. Adding a few drops of carbolic acid prevents the formation of mold.
6) Aniline blue orange, Halmi-Koneczny
0.9 g of aniline blue orange (1A-476) are dissolved in 100 cc of distilled water by heating and 1 cc of 98% glacial acetic acid is added to the hot solution. Filter after cooling.
7) Aniline blue orange, Heidenhain
2.5 g of aniline blue orange (1A-352) are dissolved in 100 cc of distilled water by heating.
8 cc of 98% acetic acid are added to the solution while it is still hot and filtered after cooling. 8) Aniline blue-orange, Mallory 4.5 g of aniline blue-orange (1A-550) are to be dissolved in 100 cc of distilled water by boiling. After cooling, filter. 9) Aniline water-gentian violet, Gram 2 cc of aniline are added to 15 cc of methyl alcohol. This mixture must remain clear. Add 85 cc of distilled water and 2 g of gentian violet (1B-411), bring to the boil and filter after cooling. 10) Aniline water-saffranin
Mix 20 cc of methyl alcohol, 2 cc of aniline and 80 cc of distilled water and shake until all the aniline is dissolved. Then add 1.5 g of safranin O (1B-463), bring to the boil briefly and filter after cooling.
11) Aniline-xylene
300 cc of pure aniline and 700 cc of xylene are mixed and stored in a dark bottle.
12) Azangemisch, Halmi-Koneczny
Solution instructions see no. 6
13) Azangemisch, Heidenhain
Solution instructions see no. 7
14) Azur-Eosin, Giemsa
To prepare the Giemsa stock solution, dissolve 0.76 g of Azur-Eosin (1A-174) in 50 cc of methyl alcohol and 50 cc of glycerin with a specific gravity of 1.23 while heating to 60 °C. The methyl alcohol and glycerin must be absolutely free of acid and water. The cooled solution is filtered through a dry filter into a dry bottle.
15) Barlow's trichrome stain
1.55 g of Barlow's trichrome (1A-522) are dissolved in 60 cc of dist. Dissolve by boiling water to which 0.25 cc of concentrated nitric acid has been added. After cooling, add 60 cc of glycerine and filter the solution.
16) Bieosinate, Tribondeau
0.2 g of Bieosinate (1A-462) are dissolved in 50 cc of glycerine and 50 cc of methyl alcohol by boiling and filtered after cooling.
17) Bismarck brown, concentrated aqueous
1 g of Bismarck brown (1B-261) is boiled with 10 cc of methyl alcohol and 90 cc of distilled water and filtered after cooling.
18) Bonner mixture
For manufacturing instructions see no. 39
19) Borax carmine, alcoholic, Grenacher
Dissolve 4 g of borax carmine (1A-434) in 100 cc of distilled water by boiling, then add 100 cc of alcohol and filter the cooled solution until it runs clear.
20) Borax carmine, aqueous, Grenacher
Dissolve 4 g of borax carmine (1A-434) in 100 cc of distilled water by boiling and filter after cooling.
21) Borax methylene blue, Manson
Dissolve 7 g of borax methylene blue (1A-354) in 100 cc of distilled water. Water by heating to around 60 °C for 10-15 minutes, add the evaporated water and filter.
22) Borax methylene blue, Unna-Sahli
G borax methylene blue (1A-480) are dissolved in 100 cc of distilled water by heating to around 60 °C for 10-15 minutes and then filtered after the evaporated water has been added.
23) Borrel eosinate
0.1 g borrel eosinate (1A-546) are dissolved in 100 cc of distilled water by gently heating and filtered after cooling (shelf life limited).
24) Boric acid methylene blue, Manson black
3 g of boric acid methylene blue (1A-432) are dissolved in 100 cc of distilled water. The water used must be boiled and cooled before use.
Dissolve 0.2 g of sodium hydroxide in 100 cc of boiled and cooled distilled water. To use, mix 10 drops of solution I) and 6 drops of solution II) and fill up to 10 cc with distilled water.
25) Brilliant cresyl blue, alcoholic
1 g of brilliant cresyl blue (1B-519) is dissolved in 100 cc of methyl alcohol while warming slightly.
26) Brilliant cresyl blue, Bender-Hettche A) Stock solution
Mix 0.850 g of sodium chloride, 100 cc of distilled water and 7 g of brilliant cresyl blue (1B-519), shake frequently (do not heat). B) Working solution 10 cc of stock solution are mixed with 40 cc of 0.85% saline solution.
27) Bunge's stain
20 g of tannin are dissolved in 100 ml of distilled water by heating. Then 1 cc of iron-III-chloride solution DAB 6 and 10 cc of a 1% aqueous fuchsin solution are added. The finished solution should look black-violet. If it appears too red, add a few more drops of iron salt solution; if it appears pure black, add a few more drops of fuchsin solution.
28) Carbol-Fuchsin, Ziehl-Neelsen
To prepare a concentrated solution, dissolve 1.6 g of Carbol-Fuchsin (1A-586) in 90 cc of distilled water and 10 cc of alcohol while heating and filter off any residue.
29) Carbol-Gentian Violet, Gram
Dissolve 0.6 g of Carbol-Gentian Violet (1A556) in 95 cc of distilled water and 25 cc of alcohol while heating and filter after cooling.
30) Carbol-Methylene Blue
Dissolve 2 g of Carbol-Methylene Blue (1A-576) in 100 cc of distilled water and filter after cooling.
31) Carbol-methyl green pyronine, Unna-Pappenheim
The solution is prepared by dissolving 1.25 g of carbol-methyl green pyronine (1A460) in 80 cc of distilled water, 16 cc of glycerine and 2 cc of alcohol while heating. The solution must be filtered.
32) Carbol-methyl green pyronine, Schenk
1.3 g of carbol-methyl green pyronine (1A-492) are dissolved in 80 cc of distilled water, 12 cc of glycerine and 4 cc of methyl alcohol while heating and filtered after cooling.
33) Carbol-methyl violet-methylene blue
For solution instructions see No. 189 A)
34) Carbol-thionin, Nicolle
Dissolve 0.150 g of thionin (1A-422) in 50 cc of alcohol by shaking. After dissolving, make up to 100 cc with 2% phenol water.
35) Carmalaun, Mayer
Dissolve 5.6 g of carmalaun (1A-334) in 100 cc of distilled water while boiling. The cooled solution is filtered.
36) Carmin, Best
Dissolve 11.5 g of carmine (1A-358) in 100 cc of distilled water while boiling. Water, add 3 cc of ammonia (specific gravity 0.96) after cooling and filter.
37) Carmine-acetic acid, Schneider
45 cc of glacial acetic acid, 55 cc of distilled water and 0.5 g of carmine (5A-176) are boiled for 1/2 to 1 hour on a reflux condenser. After cooling, the residue is filtered off.
38) China blue, Oelze
5 g of China blue (1B-507) are dissolved in 100 cc of distilled water while boiling and filtered through cotton wool after cooling.
39) Chromium-osmium-acetic acid (Bonn mixture)
180 cc of chromic acid solution 1%, 25 cc of osmic acid solution 2%, 12 cc of glacial acetic acid and 210 cc of distilled water are mixed. The solution should be stored in a dark bottle.
40) Chrysoidin, Neißer
For solution instructions see No. 135 III)
41) Crystal violet, concentrated aqueous
2 g of crystal violet (1B-345) are dissolved in 10 cc of alcohol and 90 cc of distilled water by heating. After cooling, filter.
42) Cyanoquine, Eisenberg
Dissolve 5 g of cyanoquine (1A-448) in 100 cc of distilled water. Water by boiling, allowing to settle and carefully filtering. It is advisable to always prepare only small quantities, as the solution tends to form mold.
43) Dahlia methyl green, roux
2.2 g of Dahlia methyl green (1B-531) are dissolved in 100 cc of distilled water and 10 cc of alcohol while heating.
44) Duro-Picro-Fuchsin
1.25 g of Duro-Picro-Fuchsin (1A-538) are dissolved by boiling in 100 cc of distilled water. This produces more lightfast colors than with ordinary picro-fuchsin solution according to van Gieson.
45) EA 31, 36, 50, 65, Papanicolaou
For solution instructions see No. 151-154
46) Ehrlich's triacid
For solution instructions see No. 195
47) Iron hematoxylin
5 g iron hematoxylin (1A-348) are dissolved in 90 cc of distilled water by boiling for about 1 minute (no longer). After cooling, 10 cc of ethyl alcohol are added. The filtered and tightly sealed solution can be kept for at least 14 days.
48) Iron hematoxylin, Heidenhain
2.5 g iron alum (3G-095) are dissolved in 100 cc of distilled water.
1 g of hematoxylin (5B-535) is dissolved in 10 cc of ethyl alcohol and 90 cc of distilled water.
49) Iron hematoxylin, Weigert
1 g of hematoxylin (5B-535) is dissolved in 100 cc of ethyl alcohol.
4 cc of ferrous sesquichlorate DAB 6 (3L-007) are mixed with 95 cc of distilled water and 1 cc of 25% hydrochloric acid DAB 6. Before use, equal parts A) and B) are mixed.
50) Iron trioxyhaematein, Hansen
10 g of ferric ammonium sulfate (3G-095) are dissolved in 150 cc of distilled water while warming.
1.6 g of haematoxylin (5B-535) are dissolved in 75 cc of distilled water by heating.
As soon as both solutions have cooled to room temperature, pour a) into b) while stirring constantly. Not the other way around! The solution first turns brown, then blue, then dark purple. Now heat it very gently until the solution turns brown again. Olive green solutions are over-oxidized. Reduce them again by adding 10% oxalic acid solution drop by drop to the warmed solution until the brown color is restored. The finished solution should react acidic, otherwise acidify with a few drops of oxalic acid solution.
51) Eosin, conc. alcoholic
1 g Eosin BA (1B-421) is dissolved in 70 cc methyl alcohol and 30 cc distilled water.
52) Eosin, concentrated aqueous
1 g Eosin (1B-425) is dissolved in 100 cc distilled water while heating and filtered.
53) Eosin-Hematoxylin, Ehrlich
For solution instructions see No. 90
54) Eosin-Methylene Blue, Mann
0.4 g Eosin-Methylene Blue (1A-114) is to be dissolved in 90 cc distilled water while heating.
55) Eosin methylene blue, Jenner
0.25 g of eosin methylene blue (1A-170) is dissolved in 100 cc of pure anhydrous methyl alcohol by heating to 60 °C and filtered through a dry filter into a dry bottle. The solution should be kept tightly closed.
56) Eosin methylene blue, Leishman
0.15 g of eosin methylene blue (1A-322) is dissolved in 100 cc of pure anhydrous methyl alcohol by heating to 60 °C and filtered through a dry filter into a dry bottle after cooling. Store tightly closed and away from bottles containing acid.
57) Eosin methylene blue, May-Grünwald
0.25 g of eosin methylene blue (1A-172) is dissolved in 100 cc of pure anhydrous methyl alcohol by heating to 60 °C and filtered through a dry filter into a dry bottle. The solution should be kept tightly closed.
58) Eosin methylene blue, Reuter
The solution is prepared in the same way as described under no. 57.
59) Eosin methylene blue, Wright
The solution is prepared by heating 0.2 g of eosin methylene blue (1A-324) in 100 cc of pure anhydrous methyl alcohol to 60 °C. After cooling, filter through a dry filter into a dry bottle. Keep tightly closed and away from bottles containing acid.
60) Flemming's - Chromosmium acetic acid
Manufacturing instructions see no. 39
61) Flemming's - Gentian violet
Dissolution instructions see no. 74
62) Flemming's - Orange
Dissolution instructions see no. 141
63) Flemming's - Safranin
Dissolution instructions see no. 180
64) Flemming's, Weigert
Dissolution instructions see no. 175
65) Fuchsin, concentrated alcoholic
3 g of fuchsin (1A-308) are dissolved in 80 cc of alcohol and 20 cc of distilled water while heating.
66) Fuchsin, concentrated aqueous
1 g of fuchsin (1A-308) is dissolved in 20 cc of alcohol and 80 cc of distilled water by heating and filtered after cooling.
67) Fuchsin S - methylene blue
Dissolve 0.2 g of fuchsin S - methylene blue (1A-512) in 100 cc of distilled water and filter after slightly heating and cooling.
68) Fuchsin-Ponceau, Masson
1 g of fuchsin-Ponceau (1A-350) is dissolved in 100 cc of distilled water by heating. Filtered after cooling.
69) Gallocyanin chrome alum, Einarson
5 g gallocyanin chrome alum (1A-458) are dissolved in 100 cc of distilled water by boiling and shaking frequently and then filtered after cooling.
70) Bunge's flagellum mordant
For manufacturing instructions see no. 27
71) Löffler's flagellum mordant
For manufacturing instructions see no. 107
72) Gentian violet, concentrated alcoholic
2 g gentian violet (1B-411) are dissolved in 100 cc of 70% alcohol and filtered.
73) Gentian violet, concentrated aqueous
2 g gentian violet (1B-411) are dissolved in 100 cc of distilled water. Dissolve in water by heating and filter after cooling.
74) Gentian violet, Flemming
1 g of gentian violet (1B-411) is dissolved in 20 cc of methyl alcohol and 80 cc of distilled water.
75) Gentian violet, Stirling
0.5 g of gentian violet (1B-411) is dissolved in 80 cc of distilled water, 15 cc of alcohol, 5 cc of glycerine and 2 cc of aniline.
76) Gentian violet orange, Bensley
Dissolve 1.5 g of gentian violet orange (1F-269) in 100 cc of alcohol while heating slightly and filter after cooling.
77) Giemsa solution
For solution instructions see No. 14
78) van Gieson solution
For solution instructions see No. 167
79) Gram's iodine
For solution instructions see No. 94
80) Greifswalder color mixture
4 g of Greifswalder color mixture (1A-454) are dissolved in 100 cc of distilled water by boiling. The solution develops a lot of foam, so choose a sufficiently large flask. It is not filtered.
81) Haemalaun, Mayer
5 g of Haemalaun (1A-552) are dissolved in 100 cc of distilled water by boiling, the cooled solution is filtered.
82) Haemalaun, acidic, Mayer
10 g of Haemalaun (1A-528) are dissolved in 100 cc of distilled water by heating and filtered after cooling.
83) Haematoxylin, Böhmer
10 g of haematoxylin (1A-498) are dissolved in 100 cc of distilled water while boiling. After cooling, filter.
84) Haematoxylin, Delafield
2 g of haematoxylin (1A-368) are dissolved in 70 cc of distilled water, 15 cc of alcohol and 15 cc of glycerine while heating. After cooling, filter.
85) Haematoxylin, Ehrlich
2 g of haematoxylin (1A-508) are dissolved in 40 cc of distilled water, 40 cc of glycerine and 20 cc of alcohol by boiling. After boiling, add 5 cc of glacial acetic acid and filter the cooled solution.
86) Haematoxylin, Friedländer
g Haematoxylin (1A-530) are boiled in 40 cc of distilled water, 40 cc of glycerine and 20 cc of alcohol until a dark color has appeared. After cooling, filter.
87) Haematoxylin, Harris
g Haematoxylin (1A-364) are dissolved in 100 cc of distilled water by boiling and after cooling, filter.
88) Haematoxylin, Heidenhain
For manufacturing instructions see No. 48
89) Haematoxylin, Weigert
For manufacturing instructions see No. 49
90) Haematoxylin-Eosin
Dissolve 2 g of Haematoxylin-Eosin (1A-574) in 40 cc of distilled water, 40 cc of glycerine and 20 cc of alcohol by boiling, add 5 cm of glacial acetic acid and filter after cooling.
91) Hansen's iron trioxyhematein
For manufacturing instructions see No. 50
92) Hasting's stain
Dissolve 0.3 g of Hasting's stain (1A-626) in 100 cc of methyl alcohol by heating to 60 °C. dissolved in water-free solution. After cooling, filter through a dry filter into a dry bottle and store tightly closed - protected from acid vapors.
93) Jenner's dye
For solution instructions, see No. 55
94) Iodine-potassium iodide, Gram
1.5 g of Gram's iodine (3A-450) are shaken with 10 cc of hot distilled water, then topped up to 100 cc with distilled water.
95) Iodine-potassium iodide, Lugol
4.5 g of Gram's iodine (3A-450) are shaken with 10 cc of hot distilled water, then topped up to 100 cc with distilled water.
96) Kallichrom, coarse
1 g Kallichrom (1A-470) is dissolved in 100 cc of distilled water by shaking frequently without heating. The solution is not filtered.
97) Nuclear fast red aluminum sulfate
5.2 g Nuclear fast red aluminum sulfate (1A-466) are dissolved in 95 cc of distilled water by heating. After cooling, filter.
98) Nuclear ruby
Dissolve 0.1 g Nuclear ruby (1A-268) without heating in 100 cc of distilled water. After dissolving, add 1 cc of glacial acetic acid.
99) Nuclear black
6.5 g Nuclear black (1A-366) are dissolved in 60 cc of distilled water. water and 40 cc of alcohol by heating. After cooling, the residue is filtered off.
100) Cresofuchsin
1 g of cresofuchsin (1A-340) is dissolved in 100 cc of ethyl alcohol by adding 3 cc of hydrochloric acid (specific gravity 1.125) by briefly heating and after cooling, filtered.
101) Crystal violet, Neißer
For solution instructions, see No. 135 II)
102) Crystal violet, concentrated alcoholic
1 g of crystal violet (1B-345) is dissolved in 80 cc of alcohol and 20 cc of distilled water by shaking. Filter before use.
103) Crystal violet, conc. aqueous
For solution instructions see no. 41
104) Leishman's dye
For solution instructions see no. 56
105) Lithium carmine, Orth
Dissolve 2.5 g of lithium carmine (1A-386) in 100 cc of distilled water while boiling and, after cooling, filter through the same filter until the solution runs clear. Add a few drops of carbolic acid to preserve it.
106) Lithium carbonate, concentrated aqueous
Heat 2 g of lithium carbonate in 100 cc of distilled water until it boils and, after cooling, filter off the remaining residue.
107) Löffler's flagellum mordant
Dissolve 20 g of crist. ferrous sulfate in 140 cc of distilled water while heating. water. Then add 20 g of tannin while shaking constantly. After complete dissolution, add about 1 cc of a 10% alcoholic fuchsin solution. The finished solution should look black-violet. If it has become too red, correct it with a few drops of concentrated iron salt solution; if it has remained pure black, add a few drops of fuchsin solution.
108) Löffler's methylene blue, alkaline
Solution instructions see No. 117
109) Lugol's solution
Solution instructions see No. 95
110) MacNeal's tetrachrome staine
Solution instructions see No. 193
111) Mallory mixture
Solution instructions see No. 8
112) Mann's color mixture
Solution instructions see no. 54
113) Manson's methylene blue
Solution instructions see no. 24
114) Mayer's haemalaun
Solution instructions see no. 81
115) Mayer's haemalaun, acidic
Solution instructions see no. 82
116) May-Grünwald's dye
Solution instructions see no. 57
117) Methylene blue, alkaline, Löffler
0.3 methylene blue (1A-153) is dissolved in 35 cc alcohol and 65 cc dist. Dissolve in water with gentle heating and filter.
118) Methylene blue, concentrated alcoholic
3 g methylene blue (1B-429) are dissolved in 80 cc alcohol and 20 cc distilled water with heating and filtered after cooling.
119) Methylene blue, concentrated aqueous
2 g methylene blue (1B-429) are dissolved in 10 cc alcohol and 90 cc distilled water with heating and filtered after cooling.
120) Methylene blue, Borrel
1 g methylene blue (1A-468) is dissolved in 100 cc distilled water by heating. After cooling, filter.
121) Methylene blue, polychrome, Unna
1 g methylene blue (1A-554) is dissolved in 100 cc of distilled water for 10-15 minutes while heating to 60 °C. After cooling, filter.
122) Methylene blue-acetic acid, Neißer
For solution instructions, see No. 135 I)
123) Methylene blue-eosin
For solution instructions, see eosin-methylene blue
124) Methylene blue - sulfuric acid
0.2 g methylene blue (1B-429) is dissolved in 60 cc of distilled water, 16 cc of methyl alcohol, 6 cc of glycerine and 6 cc of sulfuric acid conc. DAB 6 (limited shelf life).
125) Methyl green, conc. aqueous
2 g of methyl green (1A-292) are dissolved in 20 cc of alcohol and 80 cc of distilled water by boiling. The solution is filtered after cooling.
126) Methyl green acetic acid
2 g of methyl green (1A-292) are dissolved in 100 cc of distilled water. 2 drops of glacial acetic acid are added to the solution.
127) Methyl green orange, Kardos-Pappenheim
0.92 g of methyl green orange (1F-273) are dissolved in 100 cc of 80% methyl alcohol by heating and filtered after cooling.
128) Methyl green pyronine, Pappenheim Dissolve
1 g of methyl green pyronine (1A-560) in 100 cc of distilled water. Water and 5 cc of alcohol while heating and filtered after cooling.
129) Methyl violet, concentrated alcoholic
2 g of methyl violet (1B-415) are dissolved in 70 cc of alcohol and 30 cc of distilled water and filtered.
130) Methyl violet, concentrated aqueous
2 g of methyl violet (1B-417) are dissolved in 100 cc of distilled water by heating. After cooling, filter.
131) Methyl green pyronine, Pappenheim-Unna
For solution instructions, see No. 31
132) Muchaematein, Mayer
0.3 g of Muchaematein (1A-444) are dissolved in 40 cc of glycerine and 60 cc of distilled water while heating. After cooling, filter if necessary.
133) Mucicarmine, Mayer
1.5 g mucicarmine (1A-166) are dissolved in 100 cc of 50% alcohol while boiling. After cooling, filter through a pleated filter.
134) Sodium carmine
0.5 g sodium carmine (1A-338) are dissolved in 100 cc of distilled water by boiling and filtered after cooling.
135) Neisser's solutions I, II, III, A, B
I) 0.1 g methylene blue (1B-429) are dissolved in 95 cc of distilled water and 5 cc of glacial acetic acid.
II) 0.3 g crystal violet (1B-345) are dissolved in 10 cc of alcohol and 90 cc of distilled water. water.
III) 0.3 g of chrysoidin (1B-473) are dissolved in 1 cc of alcohol and 90 cc of distilled water by boiling. 0.1 g of methylene blue (1B-429) are dissolved in 95 cc of distilled water and 5 cc of glacial acetic acid. 0.2 g of vesuvine (1B-263) are dissolved in 100 cc of distilled water by heating.
136) Neutral mixture, Ehrlich Dissolve
1.5 g of neutral mixture (1F-331) in 100 cc of distilled water and 10 cc of alcohol while heating and filter the cooled solution.
137) Neutral violet, Unna
0.5 g of neutral violet (1F-623) are dissolved in 100 cc of hot distilled water. Dissolve in 100 cc of distilled water and filter after cooling.
138) Nigrosin, Dorner Heat
100 cc of distilled water to boiling point. Add 5 g of nigrosin (1B-161) while shaking. Boil for 30 minutes and add 0.5 cc of 40% formalin. After cooling, filter through cotton wool. 139) Opal blue, Bresslau Dissolve 5 g of opal blue (1B-509) in 100 cc of distilled water by heating. After cooling, filter. 140) Opal blue-phloxine rhodamine, Bresslau Dissolve 5.48 g of opal blue-phloxine rhodamine (1A-474) in 100 cc of distilled water by heating. After cooling, it must be filtered.
141) Orange, Flemming
0.5 g of orange (1B-221) is dissolved in 90 cc of distilled water and 10 cc of alcohol by heating and filtered after cooling.
142) Orange OG 3, Papanicolaou
For solution instructions, see No. 149
143) Orange OG 6, Papanicolaou
For solution instructions see No. 150
144) Orange - Tannin, Unna
25 g of tannin are dissolved in 75 cc of distilled water. After dissolving, add 0.5 g of Orange G (1B-221), boil and filter after cooling.
145) Orcein-acid fuchsin, Unna
1 g of orcein (1F-337) and 0.1 g of acid fuchsin (1B-525) are dissolved in 60 cc of alcohol, 10 cc of glycerine and 30 cc of distilled water by boiling. After boiling, add 2 cc of hydrochloric acid DAB 6.
146) Orth's Lithium Carmine
For solution instructions see No. 105
147) Panchrom, Laveran
0.15 g Panchrom (1F-247) are dissolved in 100 cc of methyl alcohol by heating. After cooling, the solution is filtered.
148) Panchrom, Pappenheim
0.75 g Panchrom (1A-484) are dissolved in a mixture of 50 cc of puriss methyl alcohol, 40 cc of acid-free glycerine and 10 cc of acetone by heating to 60 °C. After cooling, filter through a dry filter into a dry bottle and store tightly closed.
149) Papanicolaou, Orange OG 3
0.385 g Papanicolaou OG 3 (1A-630) are dissolved in 100 cc of 95% alcohol by heating. After cooling, filter through multiple filters.
150) Papanicolaou, Orange OG 6
0.375 g Orange OG 6 (1A-556) are dissolved in 100 cc of 95% alcohol by heating. After cooling, filter through the same filter multiple times until the solution runs clear.
151) Papanicolaou stain EA 31
0.58 g Polychrom EA 31 (1A-344) are dissolved in 95 cc of methyl alcohol and 5 cc of distilled water by boiling and filtered after cooling.
152) Papanicolaou stain EA 36
0.7 g Polychrom EA 36 (1A-124) are dissolved in 100 cc of 95% alcohol by heating and filtered after cooling.
153) Papanicolaou stain EA 50
0.71 g Polychrom EA 50 (1A-126) are dissolved in 95 cc of alcohol and 5 cc of distilled water by heating and filtered after cooling.
154) Papanicolaou stain EA 65
0.59 g Polychrom EA 65 (1A-346) are dissolved in 100 cc of 95% alcohol by boiling and filtered after cooling.
155) Pappenheim-Unna's color solution
Solution instructions see No. 31
156) Paracarmine, Mayer
5.5 g of paracarmine (1F-243) are dissolved in 70 cc of alcohol and 30 cc of distilled water by heating and filtered.
157) Patent blue, concentrated aqueous
1 g of patent blue (1B-451) are dissolved in 10 cc of alcohol and 90 cc of distilled water and filtered after cooling.
158) Phloxinrhodamine, Bresslau
6.5 g of phloxinrhodamine (1A-342) are dissolved in 100 cc of distilled water by heating. Filter after cooling.
159) Physiological saline solution
Dissolve 1 9.0 g saline tablet (3G-076) in 1,000 cc of freshly distilled water.
160) Pianese mixture
Dissolve 0.3 g of Pianese (1A-520) in 25 cc of alcohol and 75 cc of distilled water by heating. The cooled solution is filtered.
161) Picrin-aniline blue
Dissolve 1.5 g of picrin-aniline blue (1A-540) in 100 cc of distilled water by boiling and then filtered.
162) Picrin-nigrosine
Dissolve 1.25 g of picrin-nigrosine (1A-128) in 100 cc of distilled water by boiling. Dissolve in water and filter.
163) Picrin acid fuchsin, van Gieson
For solution instructions see No. 167
164) Picro blue black
0.5 g of picrin blue black (1A-526) is to be dissolved in 72 cc of distilled water and 8 cc of alcohol by boiling.
165) Picro carmine, Ranvier
Dissolve 3 g of picrin carmine (1A-332) in 100 cc of distilled water by boiling and filter after cooling.
166) Picro carmine, Weigert
1.5 g of picro carmine (1A-472) is to be dissolved in 100 cc of distilled water by boiling. The cooled solution is filtered.
167) Picro-Fuchsin, van Gieson
1 g Picro-Fuchsin (1A-488) is dissolved in 100 cc of distilled water by boiling.
168) Picro-Indigo
Carmine 1 g Picro-Indigo Carmine (1A-122) is dissolved in 100 cc of distilled water by boiling. After cooling, the solution is filtered.
169) Picro-Orange
8.2 g Picro-Orange (1A-482) are dissolved in 80 cc of alcohol and 20 cc of distilled water while heating and filtered after cooling.
170) Polychrome Methylene Blue, Terry
0.7 g Polychrome Methylene Blue (1A-446) are boiled in 100 cc of distilled water for 2.5 minutes. After rapid cooling, 10 cc of 10% acetic acid are added and filtered.
171) Polychrome methylene blue, Unna
Solution instructions see No. 121
172) Polychrome EA 31, 36, 50, 65, Papanicolaou
Solution instructions see No. 151-154
173) Ponceau - Fuchsin, Masson
Solution instructions see No. 68
174) Buffer mixture according to Weise
2 g of buffer mixture (3G-070) are made up to 1,000 cc with freshly distilled water.
175) Resorcin-Fuchsin, Weigert
g Resorcin-Fuchsin, (1A-294) is dissolved by boiling in 100 cc of ethyl alcohol to which 3 cc of hydrochloric acid (specific gravity 1.125) have been added, and filtered after cooling.
176) Roux color mixture
For solution instructions see No. 43
177) Safranelin
g Safranelin (1A-518) is dissolved by boiling in 100 cc of ethyl alcohol after adding 2 cc of hydrochloric acid (specific gravity 1.125). Filter after cooling.
178) Safranine, conc. alcoholic
2 g of safranin (1B-477) are dissolved in 100 cc of alcohol while heating. The cooled solution is filtered.
179) Safranin, conc. aqueous
g of safranin (1B-463) are dissolved in 10 cc of alcohol and 90 cc of distilled water by boiling and filtered after cooling.
180) Safranin, Flemming
0.5 g of safranin O (1B-463) are dissolved in 20 cc of methyl alcohol, 80 cc of distilled water and 1 cc of aniline.
181) Safranin, Olt
3 g of safranin (1B-463) are dissolved in 10 cc of alcohol and 90 cc of distilled water by boiling. After cooling, filter.
182) Safranin - Aniline, Babes
Manufacturing instructions see No. 10
183) Hydrochloric acid-alcohol 3%
100 cc of 70% alcohol are mixed with 3 cc of 25% hydrochloric acid.
184) Acid fuchsin-methylene blue
Solution instructions No. 136
185) Acid fuchsin-picric acid, van Gieson
Solution instructions No. 167
186) Acid fuchsin-tannin, Unna
Dissolve 25 g of tannin in 75 cc of dist. water, add 0.5 g of acid fuchsin (1B-525), boil and filter after cooling.
187) Acid fuchsin cloth fast yellow
1.5 g of acid fuchsin cloth fast yellow (1A-542) are dissolved in 90 cc of distilled water. After dissolving, add 12 drops of glacial acetic acid.
188) Schiff's reagent
0.5 g of p-rosaniline acridine-free (1B-297) are dissolved in 15 cc of hydrochloric acid (normal), without heating. After dissolving, filter if necessary, then add 0.5 g of potassium metabisulfite (K2S2O5), dissolved in 85 cc of distilled water and leave to stand in a dark room for 24 hours. If the solution is still red, a little more potassium metabisulfite must be added. A slight yellow coloration can be removed by adding 0.3 g of activated charcoal and shaking repeatedly. The solution must react acidically. Any pink coloration that appears after filtering can be removed by adding a few drops of hydrochloric acid.
189) Schlirf solutions
A. Carbol-methyl violet-methylene blue
3 g of methyl violet 6B (1B-347) and 0.8 g of methylene blue (1B-429) are dissolved in 150 cc of methyl alcohol without heating. Then 600 cc of distilled water and
6 g of carbolic acid are added.
B. Carbol-methyl green-pyronine
Solution procedure no. 31
190) Shorr stain
1.81 g Shorr (1A-270) are dissolved by boiling in 50 cc of alcohol and 50 cc of distilled water. 1 cc of glacial acetic acid is added to the solution while it is still hot and filtered after cooling.
191) Stoltenberg's color solution
0.25 g malachite green (1B-249), 0.05 g toluidine blue (1B-481) and 0.01 g hematoxylin (5B535) are dissolved in 100 cc of distilled water by boiling. 3 cc of glacial acetic acid and 3 cc of alcohol are added to the solution while it is still hot. Filter the solution after cooling.
192) Sudan solution, Daddi
2 g Sudan II (1A-254) are boiled in 80 cc alcohol and 20 cc distilled water. After cooling completely, if necessary, top up to 100 cc with 80% alcohol and filter off the residue.
193) Tetrachrome stain, MacNeal
0.3 g Tetrachrome stain (1A-516) are dissolved in 100 cc methyl alcohol (acid-free) by heating to 60 °C. After cooling, the solution is filtered through a dry filter into a dry bottle and stored tightly closed.
194) Thymen Victoria blue, Unna
1 g Victoria blue 4 R (1B-387) is dissolved by boiling in 100 cc distilled water to which 2 drops of thymen have been added. After cooling, filter.
195) Triacid mixture, Ehrlich
2 g of triacid mixture (1A-572) are dissolved in a mixture of 35 cc of alcohol, 15 cc of glycerin (specific gravity 1.23) and 50 cc of distilled water while heating. After cooling, filter.
196) Trichrome stain, Barlow
For solution instructions, see No. 15
197) Trypan blue-Congo red, Seyderhelm
1.1 g of trypan blue-Congo red (1A-536) are dissolved in 100 cc of distilled water while heating. After cooling, filter.
198) Twort stain
1 g Twort stain (1A-534) is dissolved in 60 cc of isopropyl alcohol and 40 cc of distilled water by boiling. The solution is filtered after cooling.
199) Unna's polychrome methylene blue
For solution instructions see No. 121
200) Vesuvelin
1 g Vesuvelin (1F-237) is dissolved in 100 cc of 90% ethyl alcohol by adding 1 cc of hydrochloric acid (specific gravity 1.125) while boiling. The cooled solution is filtered.
201) Vesuvin, Neißer
For solution instructions see No. 135 B)
202) Victoria blue, Herzberg
3 g Victoria blue 4 R (1B-387) are dissolved in 100 cc of distilled water by boiling. 2.5 g of citric acid are added to the still hot solution and filtered.
202) Victoria blue, Herzberg
3 g Victoria blue 4 R (1B-387) are dissolved in 100 cc of distilled water by boiling. 2.5 g of citric acid are added to the solution while it is still hot and filtered after cooling.
203) Water blue - tannin, Unna
25 g of tannin are dissolved in 75 cc of distilled water while heating. After adding 1 g of Wasserbau (1B-499) and cooling, filter.
204) Weigert's iron hematoxylin
For preparation instructions, see No. 49
205) Weigert's elastin stain solution
For dissolving instructions, see No. 175
206) Wilson's stain solution
0.3 g Wilson's stain (1A-632) is dissolved in 100 cc methyl alcohol (acid-free) by carefully heating to 60 °C. The cooled solution is filtered through a dry filter into a dry bottle and stored tightly closed.
207) Wright's stain solution
For dissolving instructions, see No. 59
208) Ziehl-Neelsen's stain solution
For dissolving instructions, see No. 28